Journal of Histotechnology

The Histology for Spatial Biology Special Collection Series (2025)

This special collection aims to bridge the evolving synergies between histology and spatial biology. By providing a platform for best practices, innovations, and cross-disciplinary dialogue, we seek to chart the future of histotechnology that leads, transforms, and elevates scientific discovery. Spatial biology represents a potential catalyst for the transformation of histotechnology, enhancing both its intellectual impact and technical relevance in modern science. We welcome contributions that advance not only the technical and methodological aspects of spatial omics but also amplify the voices of practitioners in the field. By addressing current blind spots and leveraging practical expertise, this initiative aims to foster a robust and collaborative future for histology and spatial biology alike.

In this ongoing special edition series, JOH would like to collect commentaries, perspectives, reviews, guidelines, research articles, technical notes, protocols, and case studies, on topics including but not limited to:

1. Innovations in Sample Preparation

Advanced techniques for preserving tissue integrity and cellular context in spatial assays.

Utilization of tissue microarrays (TMAs) within spatial omics platforms for streamlined workflows.

2. Standardized Protocols

Practical guides for specimen handling, tissue processing, staining, and imaging to ensure consistency.

Strategies for accessing and utilizing biobanks to support large-cohort or multi-site studies.

3. Technological Accessibility and Scalability

Approaches to simplify and scale spatial omics technologies for broader accessibility.

Balancing cost-efficiency with data quality to expand spatial omics applications without compromising performance.

4. Single-Chance Handling Techniques

Methods for managing unique and rare clinical or environmental samples where re-testing is not feasible.

Strategies to maximize outcomes from irreplaceable specimens.

5. Tissue-Specific Methodologies

Protocols tailored to challenging tissue types, such as adipose, neural, bone, cartilage, ocular tissues, pancreas and skin.

Expanding the applicability of spatial omics techniques to a wider range of tissues including non-standard organisms, plants and fungi.

6. Validation and Reproducibility

Methods to ensure reproducibility across various spatial omics platforms and experimental conditions.

Insights into antibody validation for multiplexed panels to enhance consistency and accuracy.

7. Multiomics and Integrated Workflow

Tissue-handling methodologies that enable multiple assays (e.g., transcriptomics, proteomics, and epigenomics) from a single sample or even a single section, maximizing the utility of limited tissue specimens.

Innovative approaches for integrating histological techniques with spatial or multiomic technologies.

Practical strategies for optimizing tissue preservation, fixation, and sectioning to ensure consistency and reproducibility across omics platforms.